Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.

doi: 10.1159/000495155

Figure Lengend Snippet: Fig. 1. Upregulation of KIF15 expression in human lung tumor tissues. (a) RNA sequencing data were obtained from The Cancer Genome Atlas. Statistical differences in expression between human lung tumor tissues (T) and paired adjacent normal tissues (N) were analyzed (***P<0.001). (b) KIF15 expression was detected by immunohistochemistry. The images represent typical staining patterns. (c) Individual staining intensities are represented by the heat map (left panel). Statistical differences between T and N were quantitated (right panel) (***P<0.001). (d) Proteins were extracted and subjected to SDS-PAGE and western blotting to determine KIF15 expression levels. Beta-actin (β-actin) was used as a loading control. mRNA: messenger RNA; LSCC: lung squamous cell carcinoma; LUAD: lung adenocarcinoma.

Article Snippet: The membrane was blocked for 1 h at room temperature in 5.0% non-fat milk and then incubated with monoclonal antibody against KIF15 (dilution, 1:1000) (Proteintech, Wuhan, China; catalogue no. 55407-1-AP) or beta-actin (dilution, 1:2000) (TransGen Biotech Co., Ltd., Beijing, China) at 4°C overnight.

Techniques: Expressing, RNA Sequencing, Immunohistochemistry, Staining, SDS Page, Western Blot, Control

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.

doi: 10.1159/000495155

Figure Lengend Snippet: Fig. 2. Overall survival rates of patients with lung cancer. Kaplan-Meier curves of overall survival according to KIF15 expression in patients with (a) lung cancer (**P<0.01), (b) lung adenocarcinoma (LUAD) (***P<0.001), and (c) lung squamous cell carcinoma (LSCC). ns: not significant.

Article Snippet: The membrane was blocked for 1 h at room temperature in 5.0% non-fat milk and then incubated with monoclonal antibody against KIF15 (dilution, 1:1000) (Proteintech, Wuhan, China; catalogue no. 55407-1-AP) or beta-actin (dilution, 1:2000) (TransGen Biotech Co., Ltd., Beijing, China) at 4°C overnight.

Techniques: Expressing

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.

doi: 10.1159/000495155

Figure Lengend Snippet: Fig. 3. KIF15 k n o c k d o w n reduced cell growth in human lung adenocarcinoma cancer cells. RNA was extracted from (a) shKIF15-A549 (KIF15-silenced), (b) shKIF15- NCI-H1299 and (c) shKIF15- NCI-H226 cells and the corresponding control cells (mock and scrambled shRNA) (left panels). KIF15 expression was examined by quantitative real-time polymerase chain reaction. Knockdown levels were calculated using the 2(–ΔΔCt) method. Proteins from shKIF15-A549, shKIF15-NCI-H1299 and shKIF15-NCI-H226 cells and the corresponding control cells were extracted and subjected to SDS-PAGE and western blotting to determine KIF15 expression levels (right panels). KIF15 protein knockdown efficiency was compared to control cells. Beta-actin (β-actin) was used as a loading control. (d) shKIF15-A549, (e) shKIF15-NCI-H1299 and (f) shKIF15-NCI-H226 cells and the corresponding control cells were cultured in 96-well plates. Cell growth was analyzed using a 3-(4,5-dimethyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (***P<0.001). Data are shown as the mean ± standard deviation of quintuplicate experiments. mRNA: messenger RNA; OD490: optical densities at 490 nm; shRNA: short hairpin RNA.

Article Snippet: The membrane was blocked for 1 h at room temperature in 5.0% non-fat milk and then incubated with monoclonal antibody against KIF15 (dilution, 1:1000) (Proteintech, Wuhan, China; catalogue no. 55407-1-AP) or beta-actin (dilution, 1:2000) (TransGen Biotech Co., Ltd., Beijing, China) at 4°C overnight.

Techniques: Control, shRNA, Expressing, Real-time Polymerase Chain Reaction, Knockdown, SDS Page, Western Blot, Cell Culture, MTT Assay, Standard Deviation

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: Increased KIF15 Expression Predicts a Poor Prognosis in Patients with Lung Adenocarcinoma.

doi: 10.1159/000495155

Figure Lengend Snippet: Fig. 4. KIF15 knockdown induced G1 cell cycle arrest in human lung adenocarcinoma cancer cells. The cell cycle was examined by flow cytometry in (a) shKIF15-A549, (b) shKIF15-NCI-H1299 and (c) shKIF15- NCI-H226 cells and the corresponding control cells (mock and scramble shRNA) (**P<0.01 and ***P<0.001). The data were analyzed using Modfit LT 5.0 software (Verity Software House, Topsham, ME, USA). Data are shown as the mean ± standard deviation of at least three independent experiments. shRNA: short hairpin RNA.

Article Snippet: The membrane was blocked for 1 h at room temperature in 5.0% non-fat milk and then incubated with monoclonal antibody against KIF15 (dilution, 1:1000) (Proteintech, Wuhan, China; catalogue no. 55407-1-AP) or beta-actin (dilution, 1:2000) (TransGen Biotech Co., Ltd., Beijing, China) at 4°C overnight.

Techniques: Knockdown, Flow Cytometry, Control, shRNA, Software, Standard Deviation

Journal: iScience

Article Title: Immunomodulatory potential of in vivo natural killer T (NKT) activation by NKTT320 in Mauritian-origin cynomolgus macaques

doi: 10.1016/j.isci.2022.103889

Figure Lengend Snippet:

Article Snippet: Bio-Plex Manager Software v6.2 , Bio-Rad, Hercules, CA , Cat#171STND05.

Techniques: Recombinant, Staining, Luminex, Transfection, Stable Transfection, Software